Year: 2018 | Month: April | Volume 8 | Issue 2
Effect of Amlodipine and its Combination with L-arginine on Rat Ovarium
Abstract:
Amlodipine is a calcium channel blocker used extensively as an antihypertensive agent in female population. It is reported to possess few pleotropic effects including nitric oxide production. However, its combined effect with L-arginine ( a well known nitric oxide donor) on reproductive system is not yet reported. In the present study, experimental rats were divided into three groups and treated with normal saline, amlodipine and Amlodipine + L-arginine respectievely. The test compounds were administered for a period of 30 days followed by which the ovarian tissues were subjected to assay of antioxidant status and histopathology, whereas nitric oxide was estimated in the serum. Amlodipine treated group showed non-significant increase in nitric oxide production but did not demonstrate adverse effects on antioxidant status, oestrous cyclicity and histological structure. However, amlodipine + L-arginine combination significantly (P<0.05) elevated lipid peroxidation, SOD and nitric oxide, and significantly (P<0.05) decreased GSH concentration in the ovaries. Also, the length of oestrous cyclicity was significantly (P<0.05) increased with prolonged diestrous. The histology of the ovaries revealed increased degeneration of follicles and atresia coupled with vasodilation. Thus amlodipine and L-arginine combination adversely affected the ovaries, which could be attributed to the enhanced production of nitric oxide.
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